Lawmakers in the devolved Edinburgh assembly voted by 93 to 30 to refuse "legislative consent" for the highly-contested European Union (Withdrawal) Bill now being debated by the British parliament.
Welsh and Scottish politicians rejected a proposal previous year by Britain that devolved powers returning from the European Union after Brexit should initially pass through the national parliament.
That was described as a "power grab" by the First Ministers of Wales and Scotland, and even the Scottish Tories said they found the initial bill troublesome.
While the Scottish Parliament's refusal to back the bill will not legally prevent ministers from pressing ahead, it will increase the tensions of the impact of Brexit on devolved settlements across the UK.
Michael Russell, the Scottish government´s chief Brexit negotiator, said any attempt to use Brexit to restrict the powers of the Scottish parliament "will be noted here and across Europe".
But the Tories hit out, claiming it was "patently obvious that Nicola Sturgeon wants a political crisis to provide cover for her independence drive".
Russell has invited British cabinet minister David Lidington to Scotland for last-ditch cross-party talks to break the impasse.
Community marches for man who died during arrest Jason Rivarde, a spokesperson for the Parish Sheriff's Office. "Today is just as hard as Thursday for this family", Hilliard said. Jefferson's coroner said Robinson died of asphyxiation and had signs of trauma from pressure on his neck.
"They can not pretend that no motion has been passed".
He will call on him come to Scotland and hear "hear the concerns of all parties and to discuss with the Scottish Government and the UK Government any new ideas from any of the parties".
Such a move would not prevent the UK Government from introducing the legislation - but it would be the first time Westminster has pushed through laws against the wishes of the Scottish Parliament.
"There is a clear solution which is, as I have said and as the committee agreed last week, to simply remove [clause 15] from the bill". MSP Ash Denham said that if Ms May's government valued devolution they would remove clause 11 from the Bill.
"The danger if I am being less optimistic, or pessimistic, is that the whole process crashes before [a deal] can emerge because time is running out and the clock is ticking", Sturgeon said on Monday. While some may be outraged over the principle of legislation being imposed on Scotland after it has been specifically rejected by their MSPs, "the practical consequences may not amount to much".