The gathered crowd seemed to agree with his message with applause, whoops and a standing ovation coming from the audience.
The outburst is sure to lead to controversy in the wake of recent debates about appropriate language by entertainers, including high-profile kerfuffles involving Kathy Griffin, Michelle Wolf, Roseanne Barr, and Samantha Bee.
Those watching home, though, were perhaps left a little confused as De Niro's f-bomb, as sudden as they were, were hastily bleeped by CBS.
DeNiro has been an outspoken critic of the president. He also extended condolences to Prime Minister Justin Trudeau, after Trump criticized the Canadian leader on Twitter.
While it was hoped that the trip could be used to help defuse tensions over a brewing trade war between the two countries, Trump began firing off tweets lashing out at Trudeau nearly as soon as he left.
Uber wants to know how drunk its passengers are In theory, the system could generate an alert to drivers or match suspected drunk passengers only with specially trained drivers. This can then be matched with the user's location and the time - near a nightclub and in the early hours, for example.
While he might have expected others in Hollywood to defend him, he probably didn't expect someone to suggest President Donald Trump himself was to blame after the comments.
'I have one thing to say and that's F*** Trump, ' DeNiro boomed into the microphone.
Actor Robert De Niro used the opportunity of the Tony Award ceremony to fling insults at the USA president. "Boy, do we need that now!" While CBS was quick to catch the swear for US audiences, the word wasn't censored during the Australian broadcast.
"So when De Niro finally got the call to see Springsteen play live, he said 'yes without asking any questions".